Our mission

Innovative Augmented Intelligence, AI/Machine-Learning

We provide Augmented Intelligence & Machine-Learning technologies to change the way tumor markers are assessed.

Our mission is to perfect cancer in-vitro diagnosis by combining 25 years of experience in: Cell recognition & characterization, markers quantification embedded in Pathfinder™ proprietary technology, offering automated solutions for :

Innovation story

Pathfinder™ proprietary technology achievements

Result of 25 years of experience in automated high-throughput Cell recognition & characterization, markers quantification for:

Our team

Highly skilled multidisciplinary team. Expertise in physics, image modeling & processing algorithms, IT-robotics, cell biology, histology & cytology-pathology.

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Partners

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Scientific publications

Cossutta M., Truumees B., Papine A., Kristoffersen H., Tatarinova E., Homsy C., Nielsen S., Soussaline F. Dual Contribution of Quantitative Image Analysis for the Evaluation of HER2 IHC Staining Quality, and Interpretation of HER2 Overexpression and HER2-Low Status in Breast Carcinoma. USCAP 2024, Baltimore, Maryland, USA, March 23-28, 2024.

  • Cossutta M., Bellocq J-P., Egele C., Papine A., Tatarinova E., Socha K., Soussaline M., Homsy C., Soussaline F. Evaluation automatisée de l’indice Ki67 dans le cancer du sein et la tumeur neuroendocrine par algorithme d’analyse d’images quantitative. Carrefour Pathologie, Paris, France, November 22-24, 2023
  • Cossutta M., Bellocq J-P., Egele C., Papine A., Tatarinova E., Socha K., Soussaline M., Homsy C., Soussaline F. Détermination par immunohistochimie du statut HER2-positif, HER2-low et HER2-ultralow dans le cancer du sein par algorithme d’analyse d’images quantitative. Carrefour Pathologie, Paris, France, November 22-24, 2023
  • Cossutta M., Bellocq J-P., Egele C., Papine A., Tatarinova E., Socha K., Soussaline M., Homsy C., Soussaline F. Validation of a Quantitative Image Analysis Algorithm for Ki67 Index in Breast Cancer and Neuroendocrine Tumor. Presentation tag: Digital and Computational Pathology. 35th ECP 2023, Dublin, Ireland, September 9-13, 2023
  • Cossutta M., Bellocq J-P., Egele C., Papine A., Tatarinova E., Socha K., Soussaline M., Homsy C., Klos J., Wisniewski P., Soussaline F. Validation of a Quantitative Image Analysis Algorithm for HER2 Status Determination in Breast Cancer by Immunohistochemistry as Positive, Low or Ultra-Low. Presentation tag: Digital and Computational Pathology. 35th ECP 2023, Dublin, Ireland, September 9-13, 2023
  • Cossutta M., Røge R., Kristoffersen H., Tatarinova E., Papine A., Soussaline M., Nielsen S., Soussaline F. Digital Image Analysis as a Standardized Method for External Quality Assessment of HER2 IHC in Breast Cancer. Presentation tag: Standardisation and Quality Assurance in Digital Pathology. 19th ECDP 2023, Budapest, Hungary, June 14-17, 2023
  • Røge R., Cossutta M., Kristoffersen H., Tatarinova E., Papine A., Soussaline F., Nielsen S. Digital image analysis-aided assessment of HER2 immunohistochemistry for external proficiency. Presentation Tag: Artificial Intelligence/Machine Learning. USCAP 2023, New Orleans, USA, March 11-16, 2023
  • Cossutta M., Papine A., Homsy C., Soussaline M., Egele C., Bellocq J-P., Soussaline F. Evaluation automatisée de Ki67 et HER2 dans le cancer du sein et les tumeurs neuroendocrines par analyse d’images quantitative – Prise en compte de l’hétérogénéité tumorale et de la variabilité des immunomarquages. Carrefour Pathologie, Paris, France, November 16-18, 2022
  • Cossutta M., Papine A., Homsy C., Soussaline M., Egele C., Bellocq J-P., Soussaline F. Apports de l’analyse d’image automatisée à la quantification de Ki67 dans le cancer du sein – Expérience issue du test AFAQAP 2021 avec 55 participants. 45èmes Assises de Pathologie, May 19-20, 2022, Strasbourg, France. Pathol. 2022;42(5):410-411 doi.org/10.1016/j.annpat.2022.07.023

Claveau S., Kindermann M., Papine A., Díaz-Riascos Z.,  Xavier Délen X.,  Georges P.,  López-Alemany R., Martínez Tirado O.,  Jean-Rémi Bertrand J-R., Abasolo I., Cigler P., Treussart F. (2021) Harnessing subcellular-resolved organ distribution of cationic copolymer-functionalized fluorescent nanodiamonds for optimal delivery of active siRNA to a xenografted tumor in mice. Nanoscale Journal. Read more

Cui Z et al. (2020) Circulating tumour cells as prognosis predictive markers of neoadjuvant chemotherapy-treated breast cancer patients. J of Chemotherapy. Read more

  • Socha K., Glaser C., Bienvenu L., Tatarinova E., Papine A., Soussaline F., Soussaline M. Digital quantification of immunohistochemical markers of breast cancer in daily practice. Evaluation of IMSTAR Pathfinder Tumor-Marker Automated Image Analysis System. Pathology Carrefour 2019, Paris, France, November 4-7, 2019. Read more
  • Anderson D., Najafzadeh M., Scally A., Jacob B., Griffith J., Chaha R., Linforth R., Soussaline M., Soussaline F. (2019) Using a Modified Lymphocyte Genome Sensitivity (LGS) test or TumorScan test to detect cancer at an early stage in each individual. FASEB BioAdvances 2019;1:32-39. Read more
  • Lia Y. et al. (2018) Improvement of sensitive and specific detection of circulating tumor cells using negative enrichment and immunostaining-FISH. Clinica Chimica Acta 485 (2018) 95–102. Read more 
  • Kumar Sharma A. et al. (2018) DNA damage in mouse organs and in human sperm cells by bisphenol A. Toxicological & Environmental Chemistry Journal. Read more
  • Groult J., Homsy C., Tatarinova E., Da Meda L., Benali-Furet N., Ye F., Papine A., Uzan G., Lebbé C., Wechsler J., Soussaline F. (2018) CTCs/CTMs Automated detection after size-bazed selection from blood of patients with melanoma. 11th ISMRC, Montpellier, France, May. Read more
  • Fenech M. (2017) IMSTAR Automated Image Cytometry Micronucleus Scoring systems. HUMN project, Session “Micronuclei and other nuclear anomalies as biomarkers of genotoxicity: mechanisms and application in human in vivo studies’, 12th International Conference on Environmental Mutagenesis (ICEM), Seoul, Korea, November 12-17 th, 2017.

  • Dubus J. et al (2017) Medium throughput Comet Assay optimization and development of an automated scoring system. 12th International Comet Assay Workshop (ICAW) Congress, Pamplona, Spain, August 29-31, 2017. Read more

  • A Varinot J., Alshehhe H., Furudoi A., Soussaline F., Soussaline M., Renard-Penna R., Mozer P, Cussenot O., Comperat E. (2016) Automatisation of the Gleason Score, The End of the Dilemma? A Proposal to Increase Consensus. Clinics in Oncology Read more
  • Nersesyan A., Fenech M., Bolognesi C., Misik M., Setayesh T., Wultsch G., Bonassi S., Thomas P., Knasmüller S., (2016) Use of the lymphocyte cytokinesis-block micronucleus assay in occupational biomonitoring of genome damage caused by in vivo exposure to chemical genotoxins: Past, present and future. Mutation Research 770 (2016) 1-11. Read more
  • Erami K., Sawamoto Y., Yoshino K., Sofuni T., Papine A., Homsy C., Soussaline M., Soussaline F. (2016) Improvement of in situ slide preparation procedure for semi-automated micronucleus counting system. CORESTA, Berlin, Germany, October. Read more.
  • Fenech M. (2016) New developments in the lymphocyte cytokinesis-block micronucleus cytome assay high-content, automated analysis, to measure radiation-induced DNA damage.14th International Workshop on Radiation Damage to DNA, Melbourne, Australia, March 20–24th, 2016.
  • Soussaline F. (2016) Quantitative Cellular Imaging for Biomarkers Assessment and Validation in Oncology. Sanofi – France R&D Sciences Days 2016 – Innovative Science for patients, Paris, France, March.
  • Soussaline F., Quantitative cytogenetics analysis and DNA damage evaluation in human biomonitoring by automated high-throughput high-content Image Cytometry: Pathfinder™ system. In Proceedings of the International symposium on cytogenetic analyses for occupational monitoring, Graz, Southern Austria, September 12th, 2015, Read more.
  • Gutzkow B.K., Azqueta A., Priestley C., Graupner A., Drlickova M., Dusinska M., Brunborg G., Soussaline F., Collins A., A validation trial- testing genotoxicity of several chemicals using standard, medium- and high-throughput comet formats. In Proceedings of 11th International Comet Assay Workshop (ICAW), Antwerpen, Belgium, September 1-5th, 2015.
  • Pourrut B., Ratsizafy I., Hosmy C., Dubus J., Al Souki K., Douay F., Soussaline M., Soussaline F. (2015) Development of an automated scoring system for plant comet assay. In Proceedings of 11th International Comet Assay Workshop (ICAW), Antwerpen, Belgium, September 1-5th, 2015.
  • Benzina S., Pitaval A., Lemercier C., Lustremant C., Frouin V., Wu N., Papine A., Soussaline F., Romeo P-H., Gidrol X. (2015) A kinome-targeted RNAi-based screen links FGF signaling to H2AX phosphorylation in response to radiation. Cellular and Molecular Life Sciences (The online version of this article)
  • Jackson P., Pedersen L.M., Kyjovska Z.O., Jacobsen N.R., Vogel U., Wallin H. (2014) Analysis of DNA strand break levels by high-throughput comet assay in frozen tissues. 7 International Nanotoxicology congresses (NanoTox 2014), Antalya, Turkey, April 23-26th, 2014
  • Duffy C R.E., Zhang R., How S-E., Lilienkampf A., De Sousa P A., Bradley M. (2014) Long term mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging. Biomaterials. online version of this article
  • Ermler, S., Scholze, M. and Kortenkamp, A. (2013) Genotoxic mixtures and dissimilar action: concepts for prediction and assessment, Archives of Toxicology 1- 16.
  • Ermler, S., Scholze, M. and Kortenkamp, A. (2013) Seven benzimidazole pesticides combined at sub-threshold levels induce micronuclei in vitro, Mutagenesis 28 (4) : 417- 426.
  • Soussaline F. (2013) Lymphocyte Cytokinesis-Block MicroNucleus Cytome assay Automation by Image Cytometry. Oral presentation in the Lymphocyte micronucleus and buccal micronucleus cytome assays for Human and Environmental Monitoring HUMN and HUMNxl Workshop, ULBRA Porto Alegre, Brazil, November 11-14, 2013.
  • Soussaline F., Decordier I., Papine A., Vande Look K., Plas G., Tatarinova E., Kirsch-Volders M. (2013) Automated image analysis of the human micronucleus cytome assay for high throughput biomonitoring and in vitro genotoxicity studies: IMSTAR system. In Proceedings of 11th International Conference on Environmental Mutagens (ICEM), Foz do Iguassu, Brazil, November 3-8, 2013.
  • Soussaline F., Dumont J., Papine A., Tatarinova E., Lorge E. (2013) Validation of a new scoring method of micronucleated L5178Y cells by high throughput automated image analysis. In Proceedings and oral presentation as short talk in the Symposium  “Mechanisms of micronucleus formation by environmental and genetic factors”, chaired by Pr Michael Fenech, of 11th International Conference on Environmental Mutagens (ICEM), Foz do Iguassu, Brazil, November 3-8, 2013.
  • Machado MS., Zago LA., Villela IV., Homsy C., Papine A., Soussaline F., Henriques JAP. (2013) Comparative study of manual and Pathfinder automated analysis in GLP cytokinesis-block micronucleus assay. In Proceedings of 11th International Conference on Environmental Mutagens (ICEM), Foz do Iguassu, Brazil, November 3-8, 2013.
  • Jackson P., Pedersen L.M., Kyjovska Z.O., Jacobsen N.R., Saber A.T., Hougaard K.S., Vogel U., Wallin H. (2013) Validation of freezing tissues and cells for analysis of DNA stand break levels by comet assay. Mutagenesis vol. 28 no. 6 PP; 699-707.
  • Soussaline F., Dumont J., Papine A., Tatarinova E., Lorge E. (2013) Validation of a new scoring method of micronucleated L5178Y cells by high throughput automated image analysis. In Proceedings of German-French DNA Repair Meeting, Illkirch, France, October 7-10, 2013.
  • Jackson P. (2013) High-throughput comet analysis with fully automated scoring, using frozen samples. Presentation in the session of 10th International Comet Assay Workshop (ICAW), Porto, Portugal, September 18-20th, 2013.
  • Azqueta A., Lopez de Cerain A., Collins A. (2013) Increasing the sensitivity of the comet assay as a genotoxicity assay. Poster Minitalks presented in the session of 10th International Comet Assay Workshop (ICAW), Porto, Portugal, September 18-20th, 2013.
  • Dahl H., Graupner A., Gutzkow K. B., Brunborg G. (2013) Automated Scoring of Minigels in a 96 Format. Poster Minitalks presented in the session of 10th International Comet Assay Workshop (ICAW), Porto, Portugal, September 18-20th, 2013.
  • Azqueta A., Sanchez O., Lopez de Cerain A. (2013) Do we really need the lysis step in the standard comet assay? Poster Minitalks presented in the session of 10th International Comet Assay Workshop (ICAW), Porto, Portugal, September 18-20th, 2013.
  • Kumar Sharma A., Soussaline F., Sallette J. Jørgensen V., Homy C., Dybdahl M. (2013) The influence of the number of cells scored on the sensitivity in the comet assay. Poster Minitalks presented in the session of 10th International Comet Assay Workshop (ICAW), Porto, Portugal, September 18-20th, 2013.
  • Fenech M., Kirsch-Volders M., Rossnerova A., Sram R., Romm H., Bolognesi C., Ramakumar A., Soussaline F., Schunck C., Elhajouji A., Anwar W., Bonassi S. (2013) HUMN project initiative and review of validation, quality control and prospects for further development of automated micronucleus assays using image cytometry systems. International Journal of Hygiene and Environmental Health 216 (2013) 541-552. Read more
  • Hansen A., Corless S., Cleland A., Petrik J., Gilbert N., Bradley M. (2013) Polymers for the Cell-Specific Immobilisation of Megakaryocytic Cell Lines. Macromolecular Bioscience 2013, 13, 437-443.
  • Kumar Sharma A., Soussaline F., Sallette J. Dybdahl M. (2012). The influence of the number of cells scored on the sensitivity in the comet assay. Mutation Research: Genetic Toxicology and Environmental Mutagenesis 749 (2012) 70-75. Read more
  • Soussaline F., Decordier I., Papine A., Tatarinova E., Dumont J., Lorge E., Kirsch-Volders M. (2012) Automated image analysis of the micronucleus cytome assay for high throughput human biomonitoring and in vitro genotoxicity studies: IMSTAR system. In Proceedings of 42nd EEMS annual conference, Warsaw, Poland, September 16-20, 2012.
  • Ermler S., Scholze M., Kortenkamp A., Project T10022: The combined effects of aneugenic benzimidazoles and other aneugens which act by disrupting microtubuli assembly. Food Standards Agency, Final report, May 2012. Read more
  • Soussaline F. (2012) Criteria for automated image cytometry micronucleus scoring systems evaluation and comparison. Human Workshop: HUMN/HUMNxl Automated Micronucleus Workshop using Image Cytometry Systems, Doha, Qatar, March 26, 2012.
  • Kirsch-Volders M., Papine A., Soussaline F., Decordier I. (2012) Automated image analysis of the human micronucleus cytome assay for high throughput biomonitoring studies: IMSTAR system. Human Workshop: HUMN/HUMNxl Automated Micronucleus Workshop using Image Cytometry Systems, Doha, Qatar, March 26, 2012.
  • Soussaline F., Papine A., Tatarinova E., Decordier I., Dumont J., Azqueta-Oscoz A., Sharma A.K., Binderup M-L., Collins A., Lorge E., Kirsch-Volders M. (2012) Automated image analysis of the micronucleus cytome assay and comet assay for high throughput human biomonitoring and in vitro/in vivo genotoxicity testing in industry studies: IMSTAR system. In Proceedings of 6th International Conference on Environmental Mutagens in Human Populations, Doha, Qatar, March 26-29, 2012
  • Marin-Kuan M., Holzwarth J.A., Fuerholz A., Bezençon CI., Verguet CI., Piguet D., Guignard G., Mansourian R., Raymond F., Metairon S., Schilter B. (2012) Toxicological perspective of Nrf2 activation. In Proceedings of 51st Annual Meeting Society of Toxicology, San Francisco, USA, March 11-15, 2012.
  • Azqueta A., Meier S., Priestley C., Bjerve Gutzkow K., Brunborg G., Sallette J., Soussaline F., Collins A. (2011) The influence of scoring method on variability in results obtained with the comet assay. Mutagenesis, vol. 26 (3) pp. 393-399. Read more
  • Decordier I., Papine A., Van de Look K., Plas G., Soussaline F., Kirsch-Volders M. (2011) Automated image analysis of micronuclei by IMSTAR for biomonitoring. Mutagenesis, vol. 26 (1) pp. 163-168. Read more
  • Pernagallo S. et al. (2011) Colonising new frontiers-microarrays reveal biofilm modulating polymers. J. of Materials Chemistry, 2011, 21, 96-101.

  • Gidrol X., Haydont V., Papine A., Soussaline F., Angulo J., Meyer F., Stawiaski J., Cancel-Tassin G., Cussenot O., Frandsen M-N., Lajaunie C., Rouillier P., Vert J-P. (2010) Large scale siRNA and LNA screens identifies kinase & miRNA regulating the differentiation/proliferation balance in prostate cancer cells. In proceedings of “System biology approaches to cancer and metabolic diseases”, Day of Molecular Medicine, Stockholm, Sweden, May 20-22, 2010.
  • Emery S., Ouédraogo G., Tatarinova E., Sallette J., Soussaline F., Meunier J-R. (2010) Towards an automated scoring system for the Syrian hamster embryo assay. In Proceedings of the Society of Toxicology’s 49th Annual Meeting and ToxExpoT, Salt Lake City, Utah, USA, March 7-11, 2010.
  • Gidrol X., Haydont V., Papine A., Soussaline F., Angulo J., Meyer F., Stawiaski J., Lajaunie C., Rouillier P., Vert J.P (2010) Kinome-targeted siRNA screen identifies, kinase regulating the differentiation/proliferation balance in prostate cancer cells. In Proceedings of TOPIM (hot TOPics in Molecular Imaging) Workshop, Bardonecchia, Italy, February 7-12, 2010.